Sequencing

The cores listed below are located at Harvard Medical School, affiliated institutions or other Harvard Schools. 

Biopolymer Next/Gen Sequencing Facility at HMS

Core Instruments and AB 3730xl - Sanger DNA Sequencing and Fragment Analysis;Life Technologies Quant Studio7 - QPCR Assays;Agilent Bioanalyzer & TapeStation - DNA and RNA Quality Assessment; Hamilton StarPlus Robot - Automation Services; Illumina HiSeq2500, NextSeq 500 & MiSeq - Next-gen Sequencing and Wafergen Apollo 324 Library Construction Services; Fluidigm C1 Single Cell Autoprep - Single Cell Analysis; Luminex LS200 - XMap Assays; QiaCube - Sample Preparation (DNA and RNA); and an Oligonucleotide Ordering Program; Reagents and Supplies Stock Room Program. A Comprehensive web based Laboratory Information Management System (LIMS), provides facility users with access to order services, supplies, retrieve data, review invoices and make payments online.

Core Director - Robert Steen
Core Website - https://genome.med.harvard.edu/

DF/HCC DNA Resource Core

The DF/HCC DNA Resource Core was established in the spring of 1999 to meet a growing need for DNA sequencing services at a cost that is affordable for academic labs. The services that we offer to users within the Dana Farber Harvard Cancer Center and the broader scientific community include DNA sequencing for large- and small-scale projects and plasmid distribution. Our highest priorities are quality assurance, user support and timely request fulfillment.

Core Director - Stephanie Mohr
Operations Director - Li Chan
Core Website - http://dnaseq.med.harvard.edu/index.html

Single Cell Core at HMS

This Core supports single cell sequencing and provides resources for sequencing the transcriptomes individual cells and provides scientific advising for optimal experimental design.  The Core is partnering with the Harvard Chan Bioinformatics Core to offer support from experimental design through to data analysis.

Core Director - Sarah Boswell
Admin Contact - Katrina Rudnicki
Core Website - http://iccb.med.harvard.edu/single-cell-core

FAS Sequencing Core