Imaging/Microscopy

Cell Biology Microscopy Facility

This core provides access to advanced light microscopes for the Harvard Medical School research community, including a lattice light sheet microscope. 

Core Director - Jennifer Waters
Core Manager - Talley Lambert
Core Website - http://cbmf.hms.harvard.edu/

HMS imaging cores website: https://microscopy.hms.harvard.edu

Connectomics Core

The goal of the HMS Connectomics Core is to help researchers acquire, align, segment, proofread, and annotate large-scale maps of cell-cell interactions using volumetric electron microscopy (EM), genetically encoded cell-type-specific EM tags, trans-synaptic viral tracing, machine learning algorithms, and advanced statistical methods.
 
Core Directors - Rachel Wilson and Wei-Chung Allen Lee

Conventional Electron Microscopy Facility

The Harvard Medical School EM Facility is a fee-for-service core facility open to researchers in the HMS Quadrangle and the Longwood Medical area. The Facility provides services and supervision in Transmission Electron Microscopy.

Core Director - Maria Ericsson
Core Website - http://electron-microscopy.hms.harvard.edu/

CryoEM Center

The Harvard Cryo-Electron Microscopy Center for Structural Biology is a joint effort by Harvard Medical School, Dana-Farber Cancer Institute, Boston Children’s Hospital, and Massachusetts General Hospital to provide state-of-the-art cryo-EM instrumentation and expertise for the Harvard structural biology community. This user facility offers consultation and training by staff in specimen preparation, microscope operation, image acquisition, and data analysis. The facility houses two Mark IV Vitrobots for sample preparation and two Titan Krios electron microscopes with BioQuantum energy filters and a Talos Arctica electron microscope, all equipped with Gatan K3 direct electron detectors and Volta phase plates.

Contact email:cryoem@crystal.harvard.edu
Website: https://cryoem.hms.harvard.edu/

Drosophila RNAi Screening Center

The mission of the DRSC is to provide the community with RNAi reagent libraries and a screening platform for high-throughput screens in Drosophila cultured and primary cells. Also included at the DRSC is the Transgenic RNAi Project (TRiP). The mission of the TRiP is to generate validated in vivo fly stocks for tissue-specific gene knockdown in whole animals. We provide access to information, protocols, data and software tools via our DRSC and TRiP website and underlying FlyRNAi database. The DRSC also provides access to state-of-the-art instrumentation for high-throughput, high-content fluorescence confocal imaging.

Core Director - Stephanie Mohr
Core Website - http://fgr.hms.harvard.edu/

HMS imaging cores website: https://microscopy.hms.harvard.edu

ICCB-Longwood Screening Facility

The ICCB-Longwood Screening Facility assists investigators in carrying out high-throughput screens of chemical and functional genomics libraries to identify new tools for biological research. The ICCB-Longwood compound collection is continuously growing. Over 500,000 compounds are currently available for screening, including > 12,000 'known bioactive' compounds, many of which have been characterized in animal models or in the clinic. Multiple human and mouse whole-genome siRNA libraries, as well as miRNA mimic and inhibitor libraries, are available for RNAi screening. Arrayed, synthetic single-guide RNA libraries targeting the human draggable genome are available for CRISPR knock out screening. Laboratory automation equipment is also available for use by the community for non-screening projects. The facility employs a staff-assisted screening model.

Core Director - Jennifer Smith
Faculty Director - Caroline Shamu
Admin Contact - Crystal Bettilyon
Core Website - http://iccb.med.harvard.edu/

HMS imaging cores website: https://microscopy.hms.harvard.edu

Image and Data Analysis Core

IDAC provides image data analysis services to individual labs and core facilities at Harvard Medical School, including the ICCB-Longwood Screening Facility (ICCB-L), the Drosophila RNAi Screening Center (DRSC), and the Nikon Imaging Center (NIC).

Core Website - http://idac.hms.harvard.edu/
Contact the Core - http://idac.hms.harvard.edu/access_idac

 

Immune Imaging Core

The BL2+ Imaging Facility provides the infrastructure and expertise for In-Vivo imaging of host pathogen interactions in infected mice.

Core Director - Uli von Adrian
Core Website - https://immunology.hms.harvard.edu/resources/hms-immune-imaging

Microscopy Resources on the North Quad

The MicRoN (Microscopy Resources on the North Quad) offers dedicated expertise in imaging and experimental design in light microscopy as well as technical support and basic maintenance of our microscopes. It primarily serves Microbiology, Immunology and Genetics departments but it is open to other Quad Departments. Our core includes widefield, TIRF, single point scanning and spinning disk confocal, multiphoton and SIM-TIRF microscopes.

 

Core Director - Paula Montero-Llopis
Core Website - https://micron.hms.harvard.edu/

HMS imaging cores website: https://microscopy.hms.harvard.edu

Molecular Electron Microscopy Suite

The Molecular Electron Microscopy Suite (MEMS) at Harvard Medical School is a user resource currently available to qualified researchers affiliated with Quad-based structural biology labs at HMS. This facility offers training and supervision in negative-stain and cryo-transmission electron microscopy of biological samples. Equipment includes four transmission electron microscopes, two cryo plungers, two glow discharge units, and negative stain and cryo sample preparation areas.

Contact email: cryoem@crystal.harvard.edu
Website: https://cryoem.hms.harvard.edu/

Mouse Imaging Core at HMS

The HMS Mouse Imaging Core provides researchers with in-vivo mouse imaging capabilities for endpoint and longitudinal studies using either luminescent or fluorescent reporters in an animal biosafety level 2 environment. Staff from ICCB-Longwood oversee the IVIS (In-Vivo Imaging System) Lumina II mouse imager and its use, while the IVIS room is operated and maintained by HCCM (Harvard Center for Comparative Medicine). Staff members provide access to and training on the imager and data analysis computers, while HCCM oversees all room and animal operations including facility access, safety training and orientation, animal transfers and husbandry, and protocol approval.

Core Director - Jennifer Smith
Core Website - http://iccb.med.harvard.edu/hms-ivis

Neurobiology Imaging Facility

The facility offers a wide-range of optical imaging equipment, technical and analytical support as well as full service imaging, tissue clearing and In Situ. The imaging equipment includes whole slide scanning, live cell imaging, confocal, lightsheet and super-resolution (STORM & STED) as well as basic brightfield and widefield microscopes. The facility also offers array tomography, a newly developed imaging technique that offers multiplex, ultra-high resolution volumetric imaging with depth-independent immunofluorescent staining. 

Core Directors - Michelle Ocana and Aurelien Begue
Core Website - https://nif.hms.harvard.edu/
Services Website - https://ppms.us/hms-neuroimaging/
HMS imaging cores website: https://microscopy.hms.harvard.edu

Nikon Imaging Center at Harvard Medical School

The NIC is a core light microscopy facility developed in partnership with Nikon Instruments Inc. and Micro Video Instruments Inc. The NIC@HMS maintains a wide range of light microscopy stations, including laser scanning confocal, spinning disk confocal, TIRF, STORM super-resolution, FRAP/photoactivation and more. All users are trained to use the equipment; no prior imaging experience is necessary. The NIC@HMS also offers an extensive educational program, including hands-on workshops for theoretical and practical training on light microscopy techniques.

Core Director - Jennifer Waters
Core Website - http://nic.med.harvard.edu/

HMS imaging cores website: https://microscopy.hms.harvard.edu

Systems Biology Microscopy Facility

(SBMF) is a core facility for light microscopy developed in partnership with Nikon Instruments Inc. and Micro Video Instruments Inc. The SBMF maintains laser scanning confocals, spinning disk confocals, a total internal fluorescence system, and other basic fluorescence and transmitted light microscopes. All users are offered training to use the equipment; no prior imaging experience is necessary.

Core Director - Jennifer Waters
Core Website - http://nic.med.harvard.edu/

HMS imaging cores website: https://microscopy.hms.harvard.edu