The cores below provide helpful resources for Imaging/Microscopy projects. They are located at Harvard Medical School, affiliated institutions, or other Harvard Schools.
Array Tomography Core
Array tomography is a newly developed imaging technique that offers multiplex, ultra-high resolution volumetric imaging with depth-independent immunofluorescent staining.
Core Director - Michelle Ocana
Core Contact - Mahmoud El-Rifai
Website - https://nif.hms.harvard.edu/array-tomography-core/
Conventional Electron Microscopy Facility
The Harvard Medical School EM Facility is a fee-for-service core facility open to researchers in the HMS Quadrangle and the Longwood Medical area. The Facility provides services and supervision in Transmission Electron Microscopy.
Drosophila RNAi Screening Center
The mission of the DRSC is to provide the community with RNAi reagent libraries and a screening platform for high-throughput screens in Drosophila cultured and primary cells. Also included at the DRSC is the Transgenic RNAi Project (TRiP). The mission of the TRiP is to generate validated in vivo fly stocks for tissue-specific gene knockdown in whole animals. We provide access to information, protocols, data and software tools via our DRSC and TRiP website and underlying FlyRNAi database. The DRSC also provides access to state-of-the-art instrumentation for high-throughput, high-content fluorescence confocal imaging.
Harvard NeuroDiscovery Center Enhanced Neuroimaging Core
The Core provides access to advanced confocal, multi-photon and live cell imaging microscopes and specializes in various forms of fluorescence, brightfield, DIC (Differential Interference Contrast) and live cell imaging. The facility offers users comprehensive service on confocal imaging, protocol design and microscopy training as well as digital image analysis, including consultation and custom macro program (ImageJ and Metamorph) design.
Core Director - Dan Tom
Core Website - http://neurodiscovery.harvard.edu/enhanced-neuroimaging-core
ICCB-Longwood Screening Facility
The ICCB-Longwood Screening Facility assists investigators in carrying out high-throughput screens of chemical and RNAi libraries to identify new tools for biological research. The ICCB-Longwood compound collection is continuously growing. Over 500,000 compounds are currently available for screening, including > 12,000 'known bioactive' compounds, many of which have been characterized in animal models or in the clinic. Multiple human and mouse whole-genome siRNA libraries, as well as miRNA mimic and inhibitor libraries, are available for RNAi screening. Laboratory automation equipment is also available for use by the community for non-screening projects. The facility employs a staff-assisted screening model.
Image and Data Analysis Core
IDAC provides image data analysis services to individual labs and core facilities at Harvard Medical School, including the ICCB-Longwood Screening Facility (ICCB-L), the Drosophila RNAi Screening Center (DRSC), and the Nikon Imaging Center (NIC).
Image Management Core at HMS
The Harvard Medical School Image Management Core is a new service of HMS Research IT Solutions to help researchers manage image data and metadata. The IMC was created in response to the growing complexity and difficulty of managing research image data. The first major offering of the IMC will be a shared installation of the OMERO image management platform. The HMS IT department will install and maintain an OMERO server for use by on-quad HMS researchers. This effort builds upon the successful use of the OMERO platform by the HMS LINCS project. Initial funding for this comes from the Tools and Technology Committee, HMS IT, and the Harvard Program in Therapeutic Science (HiTS).
Immunology Imaging Core
The Division of Immunology Imaging Core offers optical microscopes, open to use to investigators in Harvard Medical School and the Harvard affiliated hospitals and institutions. The facility currently has two Olympus Confocal laser scanning microscopes (Olympus Fluoview FV1000 and Olympus FV3000) and a Nikon Ti-Eclipse Fluorescence microscope.
Core Director - Harsha Radhakrishnan
Website - http://immdiv.hms.harvard.edu/ImagingCore/Imaging.html
Microscopy Resources on the North Quad
The MicRoN (Microscopy Resources on the North Quad) offers dedicated expertise in imaging and experimental design in light microscopy and well as technical support and basic maintenance of our microscopes. It primarily serves MBIB and Genetics departments but it is open to other Quad Departments.Our core includes widefield, scanning and scanning confocal microscopes.
Mouse Imaging Core at HMS
The HMS Mouse Imaging Core provides researchers with in-vivo mouse imaging capabilities for endpoint and longitudinal studies using either luminescent or fluorescent reporters in an animal biosafety level 2 environment. Staff from ICCB-Longwood oversee the IVIS (In-Vivo Imaging System) Lumina II mouse imager and its use, while the IVIS room is operated and maintained by HCCM (Harvard Center for Comparative Medicine). Staff members provide access to and training on the imager and data analysis computers, while HCCM oversees all room and animal operations including facility access, safety training and orientation, animal transfers and husbandry, and protocol approval.
Neurobiology Imaging Facility
Nikon Imaging Center at Harvard Medical School
The NIC is a core light microscopy facility developed in partnership with Nikon Instruments Inc. and Micro Video Instruments Inc. The NIC@HMS maintains a wide range of light microscopy stations, including laser scanning confocal, spinning disk confocal, TIRF, STORM super-resolution, FRAP/photoactivation and more. All users are trained to use the equipment; no prior imaging experience is necessary. The NIC@HMS also offers an extensive educational program, including hands-on workshops for theoretical and practical training on light microscopy techniques.
Systems Biology Microscopy Facility
(SBMF) is a core facility for light microscopy developed in partnership with Nikon Instruments Inc. and Micro Video Instruments Inc. The SBMF maintains laser scanning confocals, spinning disk confocals, a total internal fluorescence system, and other basic fluorescence and transmitted light microscopes. All users are offered training to use the equipment; no prior imaging experience is necessary.