Lab Automation and Screening

Drosophila RNAi Screening Center

The mission of the DRSC is to provide the community with RNAi reagent libraries and a screening platform for high-throughput screens in Drosophila cultured and primary cells. Also included at the DRSC is the Transgenic RNAi Project (TRiP). The mission of the TRiP is to generate validated in vivo fly stocks for tissue-specific gene knockdown in whole animals. We provide access to information, protocols, data and software tools via our DRSC and TRiP website and underlying FlyRNAi database. The DRSC also provides access to state-of-the-art instrumentation for high-throughput, high-content fluorescence confocal imaging.

Core Director - Stephanie Mohr
Core Website - http://fgr.hms.harvard.edu/

ICCB-Longwood Screening Facility

The ICCB-Longwood Screening Facility assists investigators in carrying out high-throughput screens of chemical and RNAi libraries to identify new tools for biological research. The ICCB-Longwood compound collection is continuously growing. Over 500,000 compounds are currently available for screening, including > 12,000 'known bioactive' compounds, many of which have been characterized in animal models or in the clinic. Multiple human and mouse whole-genome siRNA libraries, as well as miRNA mimic and inhibitor libraries, are available for RNAi screening. Laboratory automation equipment is also available for use by the community for non-screening projects. The facility employs a staff-assisted screening model.

Core Director - Caroline Shamu
Deputy Director - Jennifer Smith
Admin Contact - Katrina Rudnicki
Core Website - http://iccb.med.harvard.edu/